Analysis of Single-Cell Chromatin Data


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Documentation for package ‘Signac’ version 1.13.0

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Signac-package Signac: Analysis of Single-Cell Chromatin Data

-- A --

AccessiblePeaks Accessible peaks
AddChromatinModule Add chromatin module
AddMotifs Add DNA sequence motif information
AddMotifs.Assay Add DNA sequence motif information
AddMotifs.ChromatinAssay Add DNA sequence motif information
AddMotifs.default Add DNA sequence motif information
AddMotifs.Seurat Add DNA sequence motif information
AddMotifs.StdAssay Add DNA sequence motif information
AggregateTiles Quantify aggregated genome tiles
AggregateTiles.ChromatinAssay Quantify aggregated genome tiles
AggregateTiles.default Quantify aggregated genome tiles
AggregateTiles.Seurat Quantify aggregated genome tiles
AlleleFreq Compute allele frequencies per cell
AlleleFreq.Assay Compute allele frequencies per cell
AlleleFreq.default Compute allele frequencies per cell
AlleleFreq.Seurat Compute allele frequencies per cell
AlleleFreq.StdAssay Compute allele frequencies per cell
Annotation Annotation
Annotation.ChromatinAssay Annotation
Annotation.Seurat Annotation
Annotation<- Annotation
Annotation<-.ChromatinAssay Annotation
Annotation<-.Seurat Annotation
AnnotationPlot Plot gene annotations
as.ChromatinAssay Convert objects to a ChromatinAssay
as.ChromatinAssay.Assay Convert objects to a ChromatinAssay
atac_small A small example scATAC-seq dataset
AverageCounts Average Counts

-- B --

BigwigTrack Plot data from BigWig files
BinarizeCounts Binarize counts
BinarizeCounts.Assay Binarize counts
BinarizeCounts.default Binarize counts
BinarizeCounts.Seurat Binarize counts
blacklist_ce10 Genomic blacklist regions for C. elegans ce10 (0-based)
blacklist_ce11 Genomic blacklist regions for C. elegans ce11 (0-based)
blacklist_dm3 Genomic blacklist regions for Drosophila dm3 (0-based)
blacklist_dm6 Genomic blacklist regions for Drosophila dm6 (0-based)
blacklist_hg19 Genomic blacklist regions for Human hg19 (0-based)
blacklist_hg38 Genomic blacklist regions for Human GRCh38
blacklist_hg38_unified Unified genomic blacklist regions for Human GRCh38
blacklist_mm10 Genomic blacklist regions for Mouse mm10 (0-based)

-- C --

CallPeaks Call peaks
CallPeaks.ChromatinAssay Call peaks
CallPeaks.default Call peaks
CallPeaks.Fragment Call peaks
CallPeaks.Seurat Call peaks
Cells.Fragment Set and get cell barcode information for a 'Fragment' object
Cells<- Set and get cell barcode information for a Fragment object
Cells<-.Fragment Set and get cell barcode information for a 'Fragment' object
CellsPerGroup Cells per group
ChromatinAssay The ChromatinAssay class
ChromatinAssay-class The ChromatinAssay class
ClosestFeature Closest Feature
ClusterClonotypes Find relationships between clonotypes
CombineTracks Combine genome region plots
ConnectionsToLinks Cicero connections to links
ConvertMotifID Convert between motif name and motif ID
ConvertMotifID.Assay Convert between motif name and motif ID
ConvertMotifID.ChromatinAssay Convert between motif name and motif ID
ConvertMotifID.default Convert between motif name and motif ID
ConvertMotifID.Motif Convert between motif name and motif ID
ConvertMotifID.Seurat Convert between motif name and motif ID
ConvertMotifID.StdAssay Convert between motif name and motif ID
CountFragments Count fragments
countOverlaps Find overlapping ranges for ChromatinAssay objects
countOverlaps-method Find overlapping ranges for ChromatinAssay objects
CountsInRegion Counts in region
coverage Coverage of a ChromatinAssay object
coverage-method Coverage of a ChromatinAssay object
CoverageBrowser Genome browser
CoveragePlot Plot Tn5 insertion frequency over a region
CreateChromatinAssay Create ChromatinAssay object
CreateFragmentObject Create a Fragment object
CreateMotifMatrix Create motif matrix
CreateMotifObject Create motif object

-- D --

DensityScatter Scatterplot colored by point density
DepthCor Plot sequencing depth correlation
disjoin Inter-range transformations for ChromatinAssay objects
disjoin-method Inter-range transformations for ChromatinAssay objects
disjointBins Inter-range transformations for ChromatinAssay objects
disjointBins-method Inter-range transformations for ChromatinAssay objects
distance Find the nearest range neighbors for ChromatinAssay objects
distance-method Find the nearest range neighbors for ChromatinAssay objects
distanceToNearest Find the nearest range neighbors for ChromatinAssay objects
distanceToNearest-method Find the nearest range neighbors for ChromatinAssay objects
DownsampleFeatures Downsample Features

-- E --

ExpressionPlot Plot gene expression
Extend Extend

-- F --

FeatureMatrix Feature Matrix
FilterCells Filter cells from fragment file
FindClonotypes Find clonotypes
FindMotifs FindMotifs
findOverlaps Find overlapping ranges for ChromatinAssay objects
findOverlaps-method Find overlapping ranges for ChromatinAssay objects
findOverlaps-methods Find overlapping ranges for ChromatinAssay objects
FindTopFeatures Find most frequently observed features
FindTopFeatures.Assay Find most frequently observed features
FindTopFeatures.default Find most frequently observed features
FindTopFeatures.Seurat Find most frequently observed features
FindTopFeatures.StdAssay Find most frequently observed features
follow Find the nearest range neighbors for ChromatinAssay objects
follow-method Find the nearest range neighbors for ChromatinAssay objects
Footprint Transcription factor footprinting analysis
Footprint.ChromatinAssay Transcription factor footprinting analysis
Footprint.Seurat Transcription factor footprinting analysis
FractionCountsInRegion Fraction of counts in a genomic region
Fragment The Fragment class
Fragment-class The Fragment class
FragmentHistogram Plot fragment length histogram
Fragments Get the Fragment objects
Fragments.ChromatinAssay Get the Fragment objects
Fragments.Seurat Get the Fragment objects
Fragments<- Get the Fragment objects
Fragments<-.ChromatinAssay Get the Fragment objects
Fragments<-.Seurat Get the Fragment objects
FRiP Calculate fraction of reads in peaks per cell

-- G --

gaps Inter-range transformations for ChromatinAssay objects
gaps-method Inter-range transformations for ChromatinAssay objects
GeneActivity Create gene activity matrix
genome Access and modify sequence information for ChromatinAssay objects
genome-method Access and modify sequence information for ChromatinAssay objects
genome<--method Access and modify sequence information for ChromatinAssay objects
GenomeBinMatrix Genome bin matrix
GetCellsInRegion Get cells in a region
GetFootprintData Get footprinting data
GetFragmentData Get Fragment object data
GetGRangesFromEnsDb Extract genomic ranges from EnsDb object
GetIntersectingFeatures Find intersecting regions between two objects
GetLinkedGenes Get genes linked to peaks
GetLinkedPeaks Get peaks linked to genes
GetMotifData Retrieve a motif matrix
GetMotifData.ChromatinAssay Retrieve a motif matrix
GetMotifData.Motif Retrieve a motif matrix
GetMotifData.Seurat Retrieve a motif matrix
GetTSSPositions Find transcriptional start sites
granges Access genomic ranges for ChromatinAssay objects
granges-method Access genomic ranges for ChromatinAssay objects
granges-methods Access genomic ranges for ChromatinAssay objects
GRangesToString GRanges to String

-- H --

head.Fragment Return the first rows of a fragment file

-- I --

IdentifyVariants Identify mitochondrial variants
IdentifyVariants.Assay Identify mitochondrial variants
IdentifyVariants.default Identify mitochondrial variants
IdentifyVariants.Seurat Identify mitochondrial variants
IdentifyVariants.StdAssay Identify mitochondrial variants
InsertionBias Compute Tn5 insertion bias
InsertionBias.ChromatinAssay Compute Tn5 insertion bias
InsertionBias.Seurat Compute Tn5 insertion bias
inter-range-methods Inter-range transformations for ChromatinAssay objects
IntersectMatrix Intersect genomic coordinates with matrix rows
isCircular Access and modify sequence information for ChromatinAssay objects
isCircular-method Access and modify sequence information for ChromatinAssay objects
isCircular<--method Access and modify sequence information for ChromatinAssay objects
isDisjoint Inter-range transformations for ChromatinAssay objects
isDisjoint-method Inter-range transformations for ChromatinAssay objects

-- J --

Jaccard Calculate the Jaccard index between two matrices

-- L --

LinkPeaks Link peaks to genes
LinkPlot Plot linked genomic elements
Links Get or set links information
Links.ChromatinAssay Get or set links information
Links.Seurat Get or set links information
Links<- Get or set links information
Links<-.ChromatinAssay Get or set links information
Links<-.Seurat Get or set links information
LookupGeneCoords Get gene coordinates

-- M --

MatchRegionStats Match DNA sequence characteristics
Motif The Motif class
Motif-class The Motif class
MotifCounts Count fragments surrounding motif sites
MotifPlot Plot DNA sequence motif
Motifs Get or set a motif information
Motifs.ChromatinAssay Get or set a motif information
Motifs.Seurat Get or set a motif information
Motifs<- Get or set a motif information
Motifs<-.ChromatinAssay Get or set a motif information
Motifs<-.Seurat Get or set a motif information

-- N --

nearest Find the nearest range neighbors for ChromatinAssay objects
nearest-method Find the nearest range neighbors for ChromatinAssay objects
nearest-methods Find the nearest range neighbors for ChromatinAssay objects
NucleosomeSignal NucleosomeSignal

-- P --

PeakPlot Plot peaks in a genomic region
PlotFootprint Plot motif footprinting results
precede Find the nearest range neighbors for ChromatinAssay objects
precede-method Find the nearest range neighbors for ChromatinAssay objects

-- R --

range Inter-range transformations for ChromatinAssay objects
range-method Inter-range transformations for ChromatinAssay objects
ReadMGATK Read MGATK output
reduce Inter-range transformations for ChromatinAssay objects
reduce-method Inter-range transformations for ChromatinAssay objects
RegionHeatmap Region heatmap
RegionMatrix Region enrichment analysis
RegionMatrix.ChromatinAssay Region enrichment analysis
RegionMatrix.default Region enrichment analysis
RegionMatrix.Seurat Region enrichment analysis
RegionPlot Region plot
RegionStats Compute base composition information for genomic ranges
RegionStats.ChromatinAssay Compute base composition information for genomic ranges
RegionStats.default Compute base composition information for genomic ranges
RegionStats.Seurat Compute base composition information for genomic ranges
RunChromVAR Run chromVAR
RunChromVAR.ChromatinAssay Run chromVAR
RunChromVAR.Seurat Run chromVAR
RunSVD Run singular value decomposition
RunSVD.Assay Run singular value decomposition
RunSVD.default Run singular value decomposition
RunSVD.Seurat Run singular value decomposition
RunSVD.StdAssay Run singular value decomposition
RunTFIDF Compute the term-frequency inverse-document-frequency
RunTFIDF.Assay Compute the term-frequency inverse-document-frequency
RunTFIDF.default Compute the term-frequency inverse-document-frequency
RunTFIDF.Seurat Compute the term-frequency inverse-document-frequency
RunTFIDF.StdAssay Compute the term-frequency inverse-document-frequency

-- S --

seqinfo Access and modify sequence information for ChromatinAssay objects
seqinfo-method Access and modify sequence information for ChromatinAssay objects
seqinfo-methods Access and modify sequence information for ChromatinAssay objects
seqinfo<--method Access and modify sequence information for ChromatinAssay objects
seqlengths Access and modify sequence information for ChromatinAssay objects
seqlengths-method Access and modify sequence information for ChromatinAssay objects
seqlengths<--method Access and modify sequence information for ChromatinAssay objects
seqlevels Access and modify sequence information for ChromatinAssay objects
seqlevels-method Access and modify sequence information for ChromatinAssay objects
seqlevels<--method Access and modify sequence information for ChromatinAssay objects
seqnames Access and modify sequence information for ChromatinAssay objects
seqnames-method Access and modify sequence information for ChromatinAssay objects
seqnames<--method Access and modify sequence information for ChromatinAssay objects
SetMotifData Set motif data
SetMotifData.ChromatinAssay Set motif data
SetMotifData.Motif Set motif data
SetMotifData.Seurat Set motif data
Signac Signac: Analysis of Single-Cell Chromatin Data
SplitFragments Split fragment file by cell identities
StringToGRanges String to GRanges
subset.Fragment Subset a Fragment object
subset.Motif Subset a Motif object
SubsetMatrix Subset matrix rows and columns

-- T --

theme_browser Genome browser theme
TilePlot Plot integration sites per cell
TSSEnrichment Compute TSS enrichment score per cell
TSSPlot Plot signal enrichment around TSSs

-- U --

UnifyPeaks Unify genomic ranges
UpdatePath Update the file path for a Fragment object

-- V --

ValidateCells Validate cells present in fragment file
ValidateFragments Validate Fragment object
ValidateHash Validate hashes for Fragment object
VariantPlot Plot strand concordance vs. VMR

-- misc --

[.Motif Subset a Motif object