fitKinetics {smoke}

Fit binding kinetics

Description

Fit the BLI traces with a single association-then-dissociation equation.

Usage

fitKinetics(obj)

Arguments

obj

obj of "Bli" class

Details

We fit the whole set of BLI traces with a single association-then-dissociation equation (Sun et al. 2020). The fitting model will be stored in the kinetics slot, and the status of "fitKinetics" will be set to "TRUE".

Value

S4 object of "Bli" class

Author(s)

Qingan Sun, Xiaojun Li, James C Sacchettini

Maintainer: Qingan Sun <[e-mail]>

References

Sun Q, Li X, Perez LM, Shi W, Zhang Y, Sacchettini JC (2020). “The molecular basis of pyrazinamide activity on Mycobacterium tuberculosis PanD.” Nature Communications, 11(339).

See Also

kinetics, plotKinetics, plotResiduals

Examples

bli1 <- alignLoad(bli, 180, 780)
bli2 <- doubleBlank(bli1)
bli3 <- baseline(bli2, 1080, 1260)
bli4 <- estimate(bli3)
bli5 <- fitKinetics(bli4)