baseline {smoke}

Align BLI traces with baseline step

Description

Align BLI traces with the baseline step right before the association step. The start and end times of baseline are input arguments.

Usage

baseline(obj, tStart, tEnd)

Arguments

obj

obj of "Bli" class

tStart

start time of the baseline

tEnd

end time of the baseline

Details

This step follows doubleBlank step. The output "Bli" object contains the traces aligned with baselines, and the status of "baseline" set to "TRUE". Also the traces number will be checked. If it does not equal the number of ligand concentration, a warning will be raised: "ligand concentrations mis-match trace number".

Value

S4 object of "Bli" class

Author(s)

Qingan Sun, Xiaojun Li, James C Sacchettini

Maintainer: Qingan Sun <[e-mail]>

References

Sun Q, Li X, Perez LM, Shi W, Zhang Y, Sacchettini JC (2020). “The molecular basis of pyrazinamide activity on Mycobacterium tuberculosis PanD.” Nature Communications, 11(339).

Examples

bli1 <- alignLoad(bli, 180, 780)
bli2 <- doubleBlank(bli1)
bli3 <- baseline(bli2, 1080, 1260)