calc_fup_correction {httk} | R Documentation |
Calculate the correction for lipid binding in plasma binding assay
Description
Poulin and Haddad (2012) observed "...that for
a highly lipophilic compound, the calculated
fup is by
far [less than] the experimental values observed under
in vitro conditions." Pearce et al. (2017) hypothesized that there was additional lipid
binding in vivo that acted as a sink for lipophilic compounds, reducing the
effective fup in vivo. It is
possible that this is due to the binding of lipophilic compounds on the non
plasma-side of the rapid equilibrium dialysis plates (Waters et al., 2008).
Pearce et al. (2017) compared predicted and observed
tissue partition coefficients
for a range of compounds. They showed that predictions were improved by
adding additional binding proportional to the distribution coefficient
Dow
(calc_dow
)
and the fractional volume of lipid in
plasma (Flipid).
We calculate
Flipid as the
sum of the physiological plasma neutral lipid fractional volume and 30 percent of
the plasma neutral phospholipid fractional volume. We use values
from Peyret et al. (2010) for rats and Poulin and Haddad (2012)
for humans. The estimate of 30 percent of the
neutral phospholipid volume as neutral lipid was used for simplictity's sake in
place of our membrane affinity predictor. To account for additional binding to lipid,
plasma to water partitioning
(Kplasma:water = 1/fup)
is increased as such:
Kcorrectedplasma:water = 1/fcorrectedup = 1/fin vitroup + Dow*Flipid
Usage
calc_fup_correction(
fup = NULL,
chem.cas = NULL,
chem.name = NULL,
dtxsid = NULL,
parameters = NULL,
Flipid = NULL,
plasma.pH = 7.4,
dow74 = NULL,
species = "Human",
default.to.human = FALSE,
force.human.fup = FALSE,
suppress.messages = FALSE
)
Arguments
fup |
Fraction unbound in plasma, if provided this argument overides
values from argument parameters and |
chem.cas |
Chemical Abstract Services Registry Number (CAS-RN) – if parameters is not specified then the chemical must be identified by either CAS, name, or DTXISD |
chem.name |
Chemical name (spaces and capitalization ignored) – if parameters is not specified then the chemical must be identified by either CAS, name, or DTXISD |
dtxsid |
EPA's 'DSSTox Structure ID (https://comptox.epa.gov/dashboard) – if parameters is not specified then the chemical must be identified by either CAS, name, or DTXSIDs |
parameters |
Parameters from the appropriate parameterization function for the model indicated by argument model |
Flipid |
The fractional volume of lipid in plasma (from |
plasma.pH |
pH of plasma (default 7.4) |
dow74 |
The octanol-water distribution ratio (DOW). |
species |
Species desired (either "Rat", "Rabbit", "Dog", "Mouse", or default "Human"). |
default.to.human |
Substitutes missing fraction of unbound plasma with human values if true. |
force.human.fup |
Returns human fraction of unbound plasma in calculation for rats if true. When species is specified as rabbit, dog, or mouse, the human unbound fraction is substituted. |
suppress.messages |
Whether or not the output message is suppressed. |
Details
Note that octanal:water partitioning above 1:1,000,000 (LogDow > 6) are truncated at 1:1,000,000 because greater partitioning would likely take longer than protein binding assay itself.
Value
A numeric fraction unpbound in plasma between zero and one
Author(s)
John Wambaugh
References
Pearce RG, Setzer RW, Davis JL, Wambaugh JF (2017). “Evaluation and calibration of high-throughput predictions of chemical distribution to tissues.” Journal of pharmacokinetics and pharmacodynamics, 44, 549–565.
Peyret T, Poulin P, Krishnan K (2010). “A unified algorithm for predicting partition coefficients for PBPK modeling of drugs and environmental chemicals.” Toxicology and applied pharmacology, 249(3), 197–207.
Poulin P, Haddad S (2012). “Advancing prediction of tissue distribution and volume of distribution of highly lipophilic compounds from a simplified tissue-composition-based model as a mechanistic animal alternative method.” Journal of pharmaceutical sciences, 101(6), 2250–2261.
Schmitt W (2008). “General approach for the calculation of tissue to plasma partition coefficients.” Toxicology in vitro, 22(2), 457–467.
Waters, Nigel J., et al. "Validation of a rapid equilibrium dialysis approach for the measurement of plasma protein binding." Journal of pharmaceutical sciences 97.10 (2008): 4586-4595.