armitage_estimate_sarea {httk}

Estimate well surface area

Description

Estimate geometry surface area of plastic in well plate based on well plate format suggested values from Corning. option.plastic == TRUE (default) give nonzero surface area (sarea, m^2) option.bottom == TRUE (default) includes surface area of the bottom of the well in determining sarea. Optionally include user values for working volume (v_working, m^3) and surface area.

Usage

armitage_estimate_sarea(
  tcdata = NA,
  this.well_number = 384,
  this.cell_yield = NA,
  this.v_working = NA
)

Arguments

tcdata

A data table with well_number corresponding to plate format, optionally include v_working, sarea, option.bottom, and option.plastic

this.well_number

For single value, plate format default is 384, used if is.na(tcdata)==TRUE

this.cell_yield

For single value, optionally supply cell_yield, otherwise estimated based on well number

this.v_working

For single value, optionally supply working volume, otherwise estimated based on well number (m^3)

Value

A data table composed of any input data.table tcdata with only the following columns either created or altered by this function:

Column Name	Description	Units
well_number	number of wells on plate
sarea	surface area	m^2
cell_yield	number of cells	cells
v_working	working (filled) volume of each well	uL
v_total	total volume of each well	uL

Author(s)

Greg Honda

References

Armitage JM, Arnot JA, Wania F, Mackay D (2013). “Development and evaluation of a mechanistic bioconcentration model for ionogenic organic chemicals in fish.” Environmental toxicology and chemistry, 32(1), 115–128.