ComputeAbsolute {grandR}

Compute absolute expression using ERCC spike ins

Description

Compute absolute expression in a grandR object and puts the normalized data into a new slot

Usage

ComputeAbsolute(
  data,
  dilution = 40000,
  volume = 10,
  slot = "tpm",
  name = "absolute"
)

Arguments

data

the grandR object

dilution

the dilution of the spikein transcript in the lysis reaction mix

volume

the approximate volume of the lysis chamber (nanoliters)

slot

the slot containing relative expression values

name

the name of the new slot to put absolute expression values in

Value

a new grandR object with an additional slot

See Also

relative2abs