import_blockdesigns {gcplyr}

Import blockdesigns

Description

Function to import block-shaped designs from files and return tidy designs. This function acts as a wrapper that calls read_blocks, paste_blocks, trans_block_to_wide, trans_wide_to_tidy, and separate_tidy

Usage

import_blockdesigns(
  files,
  block_names = NULL,
  block_name_header = "block_name",
  join_as_cols = TRUE,
  sep = NULL,
  values_colname = "Designs",
  into = NULL,
  keep_blocknames = !join_as_cols,
  warn_joinrows_nointo = TRUE,
  join_designs = NULL,
  ...
)

Arguments

files

A vector of filepaths relative to the current working directory where each filepath is a single plate read to be read by read_blocks.

block_names

Vector of names corresponding to each design element (each block). Inferred from filenames, if not specified.

When keep_blocknames = TRUE, the output will have a column containing these values, with the column name specified by block_name_header.

When join_as_cols = TRUE, the block_names are also used as the output column names for each separated design column.

block_name_header

When keep_blocknames = TRUE, the column name of the column containing the block_names.

join_as_cols

logical indicating whether blocks (if there are multiple) should be joined as columns (i.e. describe the same plate) in the tidy output. If FALSE, blocks are joined as rows (i.e. describe different plates) in the tidy output.

sep

If designs have been pasted together, this specifies the string they should be split apart by via separate_tidy.

values_colname

When join_as_cols = FALSE and sep is not specified, all the design values will be in a column named by values_colname. For other cases, see the Value section.

into

When sep is specified, into sets the names of the columns after splitting (see Value section for behavior when into is not set).

keep_blocknames

logical indicating whether the column containing block_names (or those inferred from file names) should be retained in the output. By default, blocknames are retained only if join_as_cols = FALSE.

warn_joinrows_nointo

logical indicating whether warning should be raised when multiple blocks are joined as rows (join_as_cols = FALSE) and sep is specified, but into is not specified.

join_designs

Deprecated, use join_as_cols instead

...

Other arguments to pass to read_blocks, paste_blocks, trans_block_to_wide, trans_wide_to_tidy, or separate_tidy.

See Details for more information

Details

Other common arguments that you may want to provide via ... include:

startrow, endrow, startcol, endcol, sheet - specifying the location of design information inside files to read_blocks.

wellnames_sep - specifying what character (or "" for none) should be used when pasting together the rownames and column names. Note that this should be chosen to match the well names in your measures.

into - specifying the column names resulting from using separate_tidy on the values_colname column.

Note that import_blockdesigns cannot currently handle metadata specified via the metadata argument of read_blocks.

If you find yourself needing more control, you can run the steps manually, first reading with read_blocks, pasting as needed with paste_blocks, transforming to tidy with trans_block_to_wide and trans_wide_to_tidy, and separating as needed with separate_tidy.

Value

A tidy-shaped data.frame containing the design information from files. This always includes a "Well" column.

If keep_blocknames = TRUE, this includes a column with the column name specified by block_name_header and containing block_names (or block names inferred from file names).

The layout of the design values varies depending on the inputs:

If join_as_cols = TRUE, each block was joined as a column, with the columns named according to block_names (or block names inferred from file names). In this case, if sep was specified, each column was split by sep into columns named by splitting the corresponding block name by sep (post-split column names can alternatively be specified directly via into).

Otherwise, when join_as_cols = FALSE, each block was joined as rows, with the column containing all design values named by values_colname. In this case, if sep was specified, that single design column was split by sep into columns named by splitting values_colname (post-split column names can alternatively be specified directly via into).