MCO {fda.usc}

Mithochondiral calcium overload (MCO) data set

Description

The mithochondiral calcium overload (MCO) was measured in two groups (control and treatment) every 10 seconds during an hour in isolated mouse cardiac cells. In fact, due to technical reasons, the original experiment [see Ruiz-Meana et al. (2000)] was performed twice, using both the "intact", original cells and "permeabilized" cells (a condition related to the mitochondrial membrane).

Format

Elements of MCO:
..$intact: fdata class object with “intact cells”curves,

• "data": Matrix of class fdata with 89 intact cells curves (rows) measured every 10 seconds during an hour in isolated mouse cardiac cell.

• "argvals", 360 discretization points from seond 0 to 3590.

• "rangeval": range("argvals").

• "names" list with: main an overall title "Control Intact Treatment", xlab title for x axis "seconds" and ylab title for y axis "Ca".

..$classintact: Factor levels of “intact cells” curves: "1" control group and "2" treatment group.

..$permea: fdata class object with “permeabilized cells” curves (whose membrane has been removed),

• "data": Matrix of class fdata with 90 permeabilizzed cells curves (rows) measured every 10 seconds during an hour in isolated mouse cardiac cell.

• "argvals", 360 discretization points from seond 0 to 3590.

• "rangeval": range("argvals").

• "names" list with: main an overall title "Control Intact Treatment", xlab title for x axis "seconds" and ylab title for y axis "Ca".

..$classpermea: Factor levels of “permeabilized cells” curves: "1" control group and "2" treatment group.

Note

The structure of the curves during the initial period (first 180 seconds) of the experiment shows a erratic behavior (not very relevant in the experiment context) during this period.

References

Ruiz–Meana M, Garcia-Dorado D, Pina P, Inserte J, Agullo L, Soler–Soler J. Cariporide preserves mitochondrial proton gradient and delays ATP depletion in cardiomyocytes during ischemic conditions. American Journal Physiology Heart Circulatori Physiology. 2003 Sep;285(3):H999–1006.

Examples

data(MCO)
names(MCO)
par(mfrow=c(1,2))
plot(MCO$intact,col=MCO$classintact)
plot(MCO$permea,col=MCO$classpermea)