process_markers {dtangle} R Documentation

## Determines number of markers n_markers, marker list mrkrs, and gamma.

### Description

Determines number of markers n_markers, marker list mrkrs, and gamma.

### Usage

process_markers(Y, pure_samples, n_markers, data_type, gamma, markers,
marker_method)


### Arguments

 Y Expression matrix. (Required) Two-dimensional numeric. Must implement as.matrix. Each row contains expression measurements for a particular sample. Each columm contains the measurements of the same gene over all individuals. Can either contain just the mixture samples to be deconvolved or both the mixture samples and the reference samples. See pure_samples and references for more details. pure_samples The pure sample indicies. (Optional) List of one-dimensional integer. Must implement as.list. The i-th element of the top-level list is a vector of indicies (rows of Y or references) that are pure samples of type i. If references is not specified then this argument identifies which rows of Y correspond to pure reference samples of which cell-types. If references is specified then this makes same idenficiation but for the references matrix instead. n_markers Number of marker genes. (Optional) One-dimensional numeric. How many markers genes to use for deconvolution. Can either be a single integer, vector of integers (one for each cell type), or single or vector of percentages (numeric in 0 to 1). If a single integer then all cell types use that number of markers. If a vector then the i-th element determines how many marker genes are used for the i-th cell type. If single percentage (in 0 to 1) then that percentage of markers are used for all types. If vector of percentages then that percentage used for each type, respectively. If not specified then top 10% of genes are used. data_type Type of expression measurements. (Optional) One-dimensional string. An optional string indicating the type of the expression measurements. This is used to set gamma to a pre-determined value based upon the data type. Valid values are for probe-level microarray as “microarray-probe”, gene-level microarray as “microarray-gene” or rna-seq as “rna-seq”. Alternatively can set gamma directly. gamma Expression adjustment term. (Optional) One-dimensional positive numeric. If provided as a single positive number then that value will be used for gamma and over-ride the value of gamma chosen by the data_type argument. If neither gamma nor data_type are specified then gamma will be set to one. markers Marker gene indices. (Optional) List of one-dimensional integer. Top-level list should be same length as pure_samples, i.e. one element for each cell type. Each element of the top-level list is a vector of indicies (columns of Y) that will be considered markers of that particular type. If not supplied then dtangle finds markers internally using find_markers. Alternatively, one can supply the output of find_markers to the markers argument. marker_method Method used to rank marker genes. (Optional) One-dimensional string. The method used to rank genes as markers. If not supplied defaults to “ratio”. Only used if markers are not provided to argument “markers”. Options are 'ratio' selects and ranks markers by the ratio of the mean expression of each gene in each cell type to the mean of that gene in all other cell types. 'regression ' selects and ranks markers by estimated regression coefficients in a series of regressions with single covariate that is indicator of each type. 'diff' selects and ranks markers based upon the difference, for each cell type, between the median expression of a gene by each cell type and the median expression of that gene by the second most highly expressed cell type. 'p.value' selects and ranks markers based upon the p-value of a t-test between the median expression of a gene by each cell type and the median expression of that gene by the second most highly expressed cell type.

[Package dtangle version 2.0.9 Index]