Neutrophils {celltrackR}

Two-Photon Data: Neutrophils responding to an infection in the ear

Description

LysM-GFP mice were infected with S. aureus on their ear, and intravitally imaged using two-photon microscopy proximal to the infection. These cells display a fairly directed kind of motion, as they move towards infection foci. For method details, see the references below.

Usage

data("Neutrophils")

Format

## 'Neutrophils' An S3 object of class "tracks"; a list with 411 elements. Each element name identifies a cell track. Each element is a matrix containing the following three columns.

t

the time (in seconds)

x

The X coordinate (in micrometers)

y

The Y coordinate (in micrometers)

Source

Data were generated in 2021 in the Mark J. Miller Lab, Department of Medicine, Washington University in St Louis, USA.

References

Lin A and Loughman JA and Zinselmeyer BH and Miller MJ and Caparon MG (2009), Streptolysin S inhibits neutrophil recruitment during the early stages of streptococcus pyogenes infection. Infect Immun, 77(11):5190–5201. doi:10.1128/IAI.00420-09

Wang B and Zinselmeyer BH and McDole JR and Gieselman PA and Miller MJ (2010), Non-invasive imaging of leukocyte homing and migration in vivo. J Vis Exp, 46:e2062. doi:10.3791/2062

Graham DB and Zinselmeyer BH and Mascarenhas F and Delgado R and Miller MJ and Swat W (2009), Itam signaling by Vav family Rho Guanine nucleotide exchange factors regulates interstitial transit rates of neutrophils in vivo. PLOS ONE, 2(2):1–5. doi:10.1371/journal.pone.0004652

Wortel IMN and Liu AY and Dannenberg K and Berry JC and Miller MJ and Textor J (2021), CelltrackR: an R package for fast and flexible analysis of immune cell migration data. ImmunoInformatics, 1-2:100003. doi:10.1016/j.immuno.2021.100003

Examples

  ## load the tracks
  data(Neutrophils)

  ## visualize the tracks (calls function plot.tracks)
  plot(Neutrophils)