BCells {celltrackR}

Two-Photon Data: B Cells in a Lymph Node

Description

GFP-labelled B cells were injected retro-orbitally in healthy CD11c-YFP mice, and intravitally imaged (using two-photon microscopy) inside a cervical lymph node. These data illustrate the characteristic "random-walk-like" motion pattern of B cells in lymph nodes. For full method details, see references below.

Usage

data("BCells")

Format

## 'BCells' An S3 object of class "tracks"; a list with 74 elements. Each element name identifies a cell track. Each element is a matrix containing the following three columns.

t

the time (in seconds)

x

The X coordinate (in micrometers)

y

The Y coordinate (in micrometers)

Source

Data were generated in 2021 in the Mark J. Miller Lab, Department of Medicine, Washington University in St Louis, USA.

References

Miller MJ and Wei SH and Parker I and Cahalan MD (2002), Two-photon imaging of lymphocyte motility and antigen response in intact lymph node. Science, 296(5574):1869–1873. doi:10.1126/science.1070051

Wortel IMN and Liu AY and Dannenberg K and Berry JC and Miller MJ and Textor J (2021), CelltrackR: an R package for fast and flexible analysis of immune cell migration data. ImmunoInformatics, 1-2:100003. doi:10.1016/j.immuno.2021.100003

Examples

  ## load the tracks
  data(BCells)

  ## visualize the tracks (calls function plot.tracks)
  plot(BCells)