lplex {beadplexr}R Documentation

LEGENDplex example data

Description

Data from a "Human Growth Factor Panel (13-plex)" LEGENDplex experiment, with 8 controls and 1 human serum samples, all in duplicates. The beads were measured on a CytoFLEX cytometer, and the fcs-files were processed using read_fcs(), with default settings.

Usage

data("lplex")

Format

A list with 18 elements. Each element is a data.frame about 5000 rows and 4 columns (the exact number varies a little due to the data acquisition):

FSC-A

The forward scatter parameter

SSC-A

The side scatter parameter

FL6-H

Intensity in the FL6 channel

FL2-H

Intensity in the FL2 channel

The list contains 8 standard samples in duplicates, and one serum sample, also in duplicate. The names of each element have the format K3 (internal panel shorthand), C:number: for standards and S:number: for serum sample, and a number indicating the replicate (1 or 2).

Source

Ulrik Stervbo, 2016, Unpublished


[Package beadplexr version 0.5.0 Index]