Tm_Wallace {TmCalculator}

Calculate the melting temperature using the 'Wallace rule'

Description

The Wallace rule is often used as rule of thumb for approximate melting temperature calculations for primers with 14 to 20 nt length.

Usage

Tm_Wallace(ntseq, ambiguous = FALSE, outlist = TRUE)

Arguments

ntseq

Sequence (5' to 3') of one strand of the DNA nucleic acid duplex as string or vector of characters (Note: Non-DNA characters are ignored by this method).

ambiguous

Ambiguous bases are taken into account to compute the G and C content when ambiguous is TRUE.

outlist

output a list of Tm and options or only Tm value, default is TRUE.

Author(s)

Junhui Li

References

Thein S L , Lynch J R , Weatherall D J , et al. DIRECT DETECTION OF HAEMOGLOBIN E WITH SYNTHETIC OLIGONUCLEOTIDES[J]. The Lancet, 1986, 327(8472):93.

Examples

ntseq = c('acgtTGCAATGCCGTAWSDBSY') #for wallace rule

out <- Tm_Wallace(ntseq,ambiguous = TRUE)
out
out$Options