new {RDML} | R Documentation |
Creates new instance of RDML
class object
Description
This function has been designed to import data from RDML v1.1 and v1.2 format
files or from xls
file generated by Applied Biosystems 7500. To
import from xls
this file have to contain Sample Setup
and
Multicomponent Data
sheets!
Arguments
filename |
|
show.progress |
|
conditions.sep |
separator for condition defined at sample name |
format |
|
Details
File format options:
- auto
Tries to detect format by extension.
.xlsx
–excel
,.xls
–abi
,.csv
–csv
, other –rdml
- abi
Reads
.xls
files generated by ABI 7500 v.2. To create such files use File>Export; check 'Sample Setup' and 'Multicomponent Data'; select 'One File'- excel
.xls
or.xslx
file with sheets 'description', 'adp', 'mdp'. See example filetable.xlsx
- csv
.csv
file with first column 'cyc' or 'tmp' and fluorescence data in other columns- rdml
.rdml
or.lc96p
files
Warning
Although the format RDML claimed as data exchange format, the specific implementation of the format at devices from real manufacturers differ significantly. Currently this function is checked against RDML data from devices: Bio-Rad CFX96, Roche LightCycler 96 and Applied Biosystems StepOne.
Author(s)
Konstantin A. Blagodatskikh <k.blag@yandex.ru>, Stefan Roediger <stefan.roediger@b-tu.de>, Michal Burdukiewicz <michalburdukiewicz@gmail.com>
Examples
## Not run:
## Import from RDML file
PATH <- path.package("RDML")
filename <- paste(PATH, "/extdata/", "lc96_bACTXY.rdml", sep ="")
lc96 <- RDML$new(filename)
## Some kind of overview for lc96
lc96$AsTable(name.pattern = sample[[react$sample$id]]$description)
lc96$AsDendrogram()
## End(Not run)