Nucleic Acid Melting Curve Analysis


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Documentation for package ‘MBmca’ version 1.0.1-3

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MBmca-package Microbead Surface Nucleic Acid Melting Curve Analysis
diffQ Calculation of the melting temperature (Tm) from the first derivative
diffQ2 Calculation of the melting temperatures (Tm, Tm1D2 and Tm2D2) from the first and the second derivative
DMP Bimodal melting curve experiment on the surface of microbeads.
DualHyb Surface melting curve data from direct hybridization experiment of short oligonucleotide probes with bimodal melting curve pattern.
MBmca Microbead Surface Nucleic Acid Melting Curve Analysis
mcaPeaks Function to estimate the approximate local minima and maxima of melting curve data.
mcaSmoother Function to pre-process melting curve data.
MFIerror Multiple comparison of the temperature dependent variance of the refMFI
MultiMelt Surface melting curve data from direct hybridization experiment of short oligonucleotides.