Sam-class {ClassComparison} | R Documentation |
Class "Sam"
Description
Implements the "Significance Analysis of Microarrays" approach to detecting differentially expressed genes.
Usage
Sam(data, classes, nPerm=100, verbose=TRUE)
## S4 method for signature 'Sam,missing'
plot(x, y, tracks=NULL, xlab='Expected T Statistics (Empirical)',
ylab='Observed T Statistics', ...)
## S4 method for signature 'Sam'
summary(object, cutoff=1, ...)
## S4 method for signature 'Sam'
selectSignificant(object, cutoff=1, ...)
## S4 method for signature 'Sam'
countSignificant(object, cutoff=1, ...)
Arguments
data |
Either a data frame or matrix with numeric values or an
|
classes |
If |
nPerm |
An integer; the number of permutations |
verbose |
A logical flag |
x |
A |
y |
Nothing, since it is supposed to be missing. Changes to the Rd processor require documenting the missing entry. |
tracks |
a numeric vector |
xlab |
Label for the x axis |
ylab |
Label for the y axis |
object |
A |
cutoff |
A numeric value |
... |
The usual extra arguments to generic functions |
Details
The SAM approach to analyzing microarray data was developed by Tusher
and colleagues; their implementation is widely available. This is an
independent implementation based on the description in their original
paper, customized to use the same interface (and thus work with
ExpressionSet
objects) used
by the rest of the ClassComparison package. The fundamental idea
behind SAM is that the observed distribution of row-by-row two-sample
t-tests should be compared not to the theoretical null distribution
but to a null distribution estimated by a permutation test. The
Sam
constructor performs the permutation test.
Value
summary
returns an object of class SamSummary
.
selectSignificant
returns a vector of logical values.
countSignificant
returns an integer.
Creating Objects
As usual, objects can be created by new
, but better methods are
available in the form of the Sam
function. The inputs to this
function are the same as those used for row-by-row statistical tests
throughout the ClassComparison package; a detailed description can be
found in the MultiTtest
class.
Slots
t.statistics
:numeric vector containing the observed t-statistics.
observed
:numeric vector containing the sorted observed t-statistics.
expected
:numeric vector of the expected distribution of t-statistics based on a permutation test.
sim.data
:numeric matrix containing all the t-statistics from all the permutations.
call
:object of class
call
specifying the function call that was used to create this object.
Methods
- summary(object, cutoff=1, ...)
Compute a summary of the object.
- plot(x, tracks=NULL, xlab='Expected T Statistics (Empirical)', ylab='Observed t Statistics', ...)
Plot the observed and expected t-statistics. The
tracks
argument causes parallel lines to be drawn on either side of the quantile-quantile central line, at the specified offsets. Colors in the plot are controlled by the current values ofoompaColor$CENTRAL.LINE
andoompaColor$CONFIDENCE.CURVE
- selectSignificant(object, cutoff=1, ...)
Compute a vector that selects significant values
- countSignificant(object, cutoff=1, ...)
Count the number of significant values
Author(s)
Kevin R. Coombes krc@silicovore.com
References
Tusher VG, Tibshirani R, Chu G.
Significance analysis of microarrays applied to the ionizing radiation
response.
Proc Natl Acad Sci U S A (2001) 98, 5116-5121.
See Also
Examples
showClass("Sam")
ng <- 10000
ns <- 50
nd <- 100
dat <- matrix(rnorm(ng*ns), ncol=ns)
dat[1:nd, 1:(ns/2)] <- dat[1:nd, 1:(ns/2)] + 2
dat[(nd+1):(2*nd), 1:(ns/2)] <- dat[(nd+1):(2*nd), 1:(ns/2)] - 2
cla <- factor(rep(c('A', 'B'), each=25))
res <- Sam(dat, cla)
plot(res)
plot(res, tracks=1:3)
summary(res)
summary(res, cutoff=2)
a <- summary(res)
plot(a@significant.calls)
plot(a@significant.calls[1:300])
countSignificant(res, 1)