import_sintax_tax_table {speedytax}

Import SINTAX Taxonomy Table

Description

Imports taxonomy files created with USEARCH and vsearch sintax commands

Usage

import_sintax_tax_table(in_file, confidence)

Arguments

in_file

A fix-rank tab-delimited text file output by SINTAX

confidence

The confidence level for filtering the taxonomy (0.8 by default)

Details

This function works with both vsearch and USEARCH sintax results.

A confidence value of 0.8 is recommended for full-length 16S rRNA gene sequences and a value of 0.5 is recommended for shorter amplicons.

Value

A phyloseq tax_table object

References

Rognes T, Flouri T, Nichols B, Quince C, Mahé F. (2016) VSEARCH: a versatile open source tool for metagenomics. PeerJ 4:e2584. doi: 10.7717/peerj.2584

Edgar RC (2016) SINTAX: a simple non-Bayesian taxonomy classifier for 16S and ITS sequences. bioRxiv. doi:10.1101/074161

Examples

# With a vsearch sintax result
taxonomy_file <- system.file("extdata", "vsearch_sintax_table.tsv", package = "speedytax")
example_tax_table <- import_sintax_tax_table(in_file = taxonomy_file)
example_tax_table
# With a USEARCH sintax result
taxonomy_file <- system.file("extdata", "usearch_sintax_table.tsv", package = "speedytax")
example_tax_table <- import_sintax_tax_table(in_file = taxonomy_file)
example_tax_table