scapeForSeurat {scaper}

Cytokine activity scores for a Seurat matrix.

Description

Computes cell-level estimates of cytokine activity for a scRNA-seq Seurat count matrix using the scapeForSeurat method. SCAPE activity estimates are computed by scoring weighted gene sets from the CytoSig or Reactome databases using the Variance-adjusted Mahalanobis (VAM) method as implemented in the VAM::vamForSeurat() function. Individual gene sets for subsequent scoring can be reconstructed using the genesetCytoSig and the genesetReactome functions for the CytoSig and the Reactome database, respectively.

Usage

scapeForSeurat(
  seurat.object,
  database = "cytosig",
  cytokine = "all",
  normalize = TRUE
)

Arguments

seurat.object

Seurat counts matrix.

database

Database used for gene set construction and set scoring.

• "cytosig" (default) performs scoring for up to 41 cytokines using the CytoSig database.

• "reactome" performs scoring for up to 30 cytokines using the Reactome database.

cytokine

Vector of cytokine names to score for activity. The default value of "all" will score all 41 cytokines supported by CytoSig or 31 supported by Reactome. Please see function supportedCytokines to view all the CytoSig or the Reactome specific scored cytokines.

normalize

Boolean indicator for whether normalization should be performed before performing gene set scoring.

Value

Seurat object consisting of cell-level cytokine activity scores returned as a separate assay (scape for scoring via the CytoSig database and VAMcdf for scoring via the Reactome database).

See Also

genesetCytoSig, genesetReactome, scape

Examples

library(SeuratObject)
CytoSig.score.output.all <- scapeForSeurat(seurat.object = pbmc_small, 
database = "cytosig", cytokine = "all", normalize=TRUE)
(as.data.frame(CytoSig.score.output.all@assays$scape@data))[1:6,1:3]
CytoSig.score.output.specific <- scapeForSeurat(seurat.object = pbmc_small, 
database = "cytosig", cytokine = c("IL4", "IL13"), normalize=TRUE)
(as.data.frame(CytoSig.score.output.specific@assays$scape@data))[,1:3]