Create_CellBender_Merged_Seurat {scCustomize} | R Documentation |
Create Seurat Object with Cell Bender and Raw data
Description
Enables easy creation of Seurat object which contains both cell bender data and raw count data as separate assays within the object.
Usage
Create_CellBender_Merged_Seurat(
raw_cell_bender_matrix = NULL,
raw_counts_matrix = NULL,
raw_assay_name = "RAW",
min_cells = 5,
min_features = 200,
...
)
Arguments
raw_cell_bender_matrix |
matrix file containing the cell bender correct counts. |
raw_counts_matrix |
matrix file contain the uncorrected Cell Ranger (or other) counts. |
raw_assay_name |
a key value to use specifying the name of assay. Default is "RAW". |
min_cells |
value to supply to min.cells parameter of |
min_features |
value to supply to min.features parameter of |
... |
Extra parameters passed to |
Value
A Seurat Object contain both the Cell Bender corrected counts ("RNA" assay) and uncorrected
counts ("RAW" assay; or other name specified to raw_assay_name
).
Examples
## Not run:
seurat_obj <- Create_CellBender_Merged_Seurat(raw_cell_bender_matrix = cb_matrix,
raw_counts_matrix = cr_matrix)
## End(Not run)
[Package scCustomize version 2.1.2 Index]