efficiency {rtpcr}

Slope, R2 and Efficiency (E) statistics and standard curves

Description

The efficiency function calculates amplification efficiency and returns related statistics and standard curves.

Usage

efficiency(df)

Arguments

df

a data frame of dilutions and Ct of genes. First column is dilutions and other columns are Ct values for different genes.

Details

The efficiency function calculates amplification efficiency of genes, and present the Slope, Efficiency, and R2 statistics.

Value

A list 3 elements.

efficiency

Slope, R2 and Efficiency (E) statistics

Slope_compare

slope comparison table

plot

standard curves

Author(s)

Ghader Mirzaghaderi

Examples

# locate and read the sample data
data_efficiency

# Applying the efficiency function
efficiency(data_efficiency)