co2_flush {respirometry}

Calculate CO2 to add to flush reservoir

Description

Calculates the moles of CO2 gas to be added to a seawater reservoir before flushing a respirometer to achieve the desired pCO2 in the respirometer after the flush. Useful for ocean acidification experiments where CO2 treatments are desired.

Usage

co2_flush(
  goal_pco2,
  resp_pH,
  resp_vol,
  flush_pH,
  flush_vol,
  flush_remain = 0,
  temp = 25,
  sal = 35,
  TA = NULL,
  atm_pres = 1013.25
)

Arguments

goal_pco2

the desired pCO2 in the respirometer after the flush (uatm).

resp_pH

pH inside the respirometer before the flush (total scale).

resp_vol

volume of the respirometer (liter).

flush_pH

pH of the reservoir water used for flushing before CO2 is added (total scale).

flush_vol

volume of the flush reservoir (liter).

flush_remain

volume of the flush reservoir that will remain after the flush (liter).

temp

temperature (°C). Default is 25 °C.

sal

salinity (psu). Default is 35 psu. If sal < 26 psu, then TA must be provided.

TA

(optional) total alkalinity (umol / kg). If undefined TA is estimated from salinity using guess_TA.

atm_pres

atmospheric pressure (mbar). Default is 1013.25 mbar.

Value

moles of CO2 gas to be added to the flush reservoir.

Note

It is assummed that the entire reservoir is drained into the respirometer during the flush. It is also assumed that all of the CO2 added dissolves and remains in solution. This can be achieved (almost completely) by bubbling CO2 according to Jokiel et al. 2014.

Author(s)

Matthew A. Birk, [e-mail]

References

Jokiel PL, Bahr KD, Rodgers KS. 2014. Low-cost, high-flow mesocosm system for simulating ocean acidification with CO2 gas. Limnol Oceanogr Methods. 12:313–322.

See Also

co2_add, co2_rate, flush_carb, carb, peri_pump

Examples

# I want the respirometer to have a pCO2 = 1000 uatm. It currently has a pH of 7.6 and is 90 L.
# If I have a 200 L reservoir with pH = 7.9 seawater, how much CO2 do I need
# to add to the flush reservoir?
co2_flush(goal_pco2 = 1000, resp_pH = 7.6, resp_vol = 90, flush_pH = 7.9, flush_vol = 200)