closed {respirometry}

Closed respirometry

Description

Returns the unknown parameter given 3 of 4 parameters to calculate respiration rate in a closed respirometer. This is useful both for basic closed respirometry setups, and also for the closed measurement phase of intermittent respirometry. Note that this is mainly useful for designing respirometry experiments. If you already have a timeseries dataset of oxygen measurements you want to analyze, use calc_MO2 instead.

Usage

closed(MO2, delta_pO2, duration, vol, temp = 25, sal = 35, atm_pres = 1013.25)

Arguments

MO2

whole-animal oxygen consumption rate (umol O2 / hour).

delta_pO2

desired change in pO2 (% air saturation).

duration

desired duration to reach delta_pO2 (minutes).

vol

volume of the respirometer (liter).

temp

temperature (°C). Default is 25 °C.

sal

salinity (psu). Default is 35 psu.

atm_pres

atmospheric pressure (mbar). Default is 1013.25 mbar.

Note

If there are more than two O2 observations, consider using calc_MO2.

Author(s)

Matthew A. Birk, [e-mail]

See Also

flush_water, calc_MO2

Examples

# I've read in the literature that my animal has an SMR of 200 umol/h. How large of a
# respirometer do I want if I want it to breathe down to 80% air saturation in 30 minutes?
closed(MO2 = 200, delta_pO2 = 100 - 80, duration = 30) # returns respirometer volume

# I've read in the literature that my animal has an SMR of 1000 umol/h. How long will it take to
# breathe down a 50 L respirometer by 10% air saturation?
closed(MO2 = 1000, delta_pO2 = 10, vol = 50) # returns the duration to breathe down the O2

# How does animal size affect how long my measurement periods last?
mass_range <- seq(100, 400, 50)
dur_range <- (closed(MO2 = scale_MO2(mass_1 = 100, MO2_1 = 400, mass_2 = mass_range),
 delta_pO2 = 20, vol = 10))
plot(mass_range, dur_range, type = 'b')

# What is the MO2 if O2 drops 0.44 mg/l in 33 minutes when the respirometer volume is 30 L?
closed(delta_pO2 = conv_o2(o2 = 0.44, from = 'mg_per_l', to = 'percent_a.s.'), duration = 33,
 vol = 30)