CalRTable {qPCRtools}

Calculate RNA volume for reverse transcription.

Description

The first step of qPCR is usually the preparation of cDNA. We need to calculate the column of RNA for reverse transcription to cDNA. So, if we have the concentration of RNA, we can use the function 'CalRTable' to do that.

Arguments

data

A data.frame contained the sample names and the concentration value. The default unit of concentration is ng/uL.

template

A data.frame contained the information of reverse transcription. In this data.frame there must be a column called 'all'.

RNA.weight

RNA weight required for reverse transcription. Default is 1 ug.

Value

A list contain a table and a figure.

Author(s)

Xiang LI <[e-mail]>

Examples

df.1.path <- system.file("examples", "crtv.data.txt", package = "qPCRtools")
df.2.path <- system.file("examples", "crtv.template.txt", package = "qPCRtools")
df.1 <- read.table(df.1.path, sep = "\t", header = TRUE)
df.2 <- read.table(df.2.path, sep = "\t", header = TRUE)
result <- CalRTable(data = df.1, template = df.2, RNA.weight = 2)
head(result)