CalExpCurve {qPCRtools} | R Documentation |
Calculate expression using standard curve.
Description
Calculate expression using standard curve.
Arguments
cq.table |
The data frame of the position and Cq value. |
design.table |
The data frame of the position and corresponding information. |
correction |
Correct expression value by reference gene. |
ref.gene |
The name of reference gene. |
stat.method |
Statistical method. |
ref.group |
The name of reference group. |
fig.type |
Output image type, 'box' represents 'boxplot', 'bar' represents 'barplot'. |
fig.ncol |
Number of columes of figure. |
Value
A list contain a table and a figure.
Author(s)
Xiang LI <lixiang117423@gmail.com>
Examples
df1.path = system.file("examples", "cal.exp.curve.cq.txt", package = "qPCRtools")
df2.path = system.file("examples", "cal.expre.curve.sdc.txt", package = "qPCRtools")
df3.path = system.file("examples", "cal.exp.curve.design.txt", package = "qPCRtools")
cq.table = read.table(df1.path, header = TRUE)
curve.table = read.table(df2.path, sep = "\t", header = TRUE)
design.table = read.table(df3.path, header = TRUE)
CalExpCurve(
cq.table,
curve.table,
design.table,
correction = TRUE,
ref.gene = "OsUBQ",
stat.method = "t.test",
ref.group = "CK",
fig.type = "box",
fig.ncol = NULL) -> res
res[["table"]]
res[["figure"]]
[Package qPCRtools version 1.0.1 Index]