CalExp2ddCt {qPCRtools}

Calculate expression using standard curve.

Description

Calculate expression using standard curve.

Arguments

cq.table

The data frame of the position and cq value.

design.table

The data frame of the position and corresponding information.

correction

Correct expression value by reference gene.

ref.gene

The name of reference gene.

ref.group

The name of reference group.

stat.method

Statistical method.

remove.outliers

Remove the outliers of each group and gene, or not.

fig.type

Output image type, 'box' represents 'boxplot', 'bar' represents 'barplot'.

fig.ncol

Number of columes of figure.

Value

A list contain a table and a figure.

Author(s)

Xiang LI <[e-mail]>

Examples

df1.path = system.file("examples", "ddct.cq.txt", package = "qPCRtools")
df2.path = system.file("examples", "ddct.design.txt", package = "qPCRtools")

cq.table = read.table(df1.path, header = TRUE)
design.table = read.table(df2.path, header = TRUE)

CalExp2ddCt(cq.table,
            design.table,
            ref.gene = "OsUBQ",
            ref.group = "CK",
            stat.method = "t.test",
            remove.outliers = TRUE,
            fig.type = "box",
            fig.ncol = NULL) -> res

res[["table"]]
res[["figure"]]