hit_grid {platetools}

Plots multiple platemaps with and identifies hits

Description

Converts numerical values and well labels into 'hits' in the form of multiple plate maps. Hits are calculated as wells above or below a specified number of standard deviations from the overall average

Usage

hit_grid(
  data,
  well,
  plate_id,
  threshold = 2,
  ncols = 2,
  plate = 96,
  each = FALSE,
  scale_each = FALSE,
  palette = "Spectral",
  ...
)

Arguments

data

Numerical values to be scaled and plotted

well

Vector of well identifiers. e.g "A01"

plate_id

Vector of plate identifiers e.g "Plate_1"

threshold

Numerical value of standard deviations from the mean for a well to be classified as a 'hit'. Default it +/- 2 SD

ncols

Number of columns in the grid of plates

plate

Number of wells in the complete plates (96, 384 or 1536)

each

boolean, allowed for backwards compatibility, scale_each is now the preferred argument name

scale_each

boolean, if true scales each plate individually, if false will scale the pooled values of data

palette

RColorBrewer palette

...

additional arguments for plot wrappers

Value

ggplot plot

Examples

df01 <- data.frame(well = num_to_well(1:96),
  vals = rnorm(96),
  plate = 1)

df02 <- data.frame(well = num_to_well(1:96),
  vals = rnorm(96),
  plate = 2)

df <- rbind(df01, df02)

hit_grid(data = df$vals,
    well = df$well,
    plate_id = df$plate,
    plate = 96,
    each = FALSE)