spots.combined {nucim} | R Documentation |
Find spots using information from two channels
Description
Find spots using information from two channels
Usage
spots.combined(
red,
green,
mask,
size = NULL,
voxelsize = NULL,
thresh.offset = 0.1,
window = c(5, 5),
min.sum.intensity = 2,
max.distance = 0.5,
use.brightest = FALSE,
max.spots = NA,
full.voxel = FALSE
)
Arguments
red |
image |
green |
image |
mask |
image mask |
size |
size of img in microns |
voxelsize |
size of voxel in microns |
thresh.offset |
Thresh offset used in EBImage::thresh() |
window |
Half width and height of the moving rectangular window. |
min.sum.intensity |
spots smaller than min.sum.intensity are ignored |
max.distance |
use only spots with distance to other color spot smaller than max.distance |
use.brightest |
Logical; use only brightest in max.distance? |
max.spots |
maximum of spots (per channel), only when use brightest=TRUE |
full.voxel |
Logical; output contains full voxel instead of rgb intensities |
Value
RGB image with spots will be written to output folder
[Package nucim version 1.0.11 Index]