normalize_pools {nanostringr}

Normalize data using common pools

Description

Normalize nanostring gene expression using common pools between two CodeSets.

Usage

normalize_pools(x, ref, x_pools, ref_pools, p = 3, weigh = TRUE)

Arguments

x

target data

ref

reference data

x_pools

target pool samples

ref_pools

reference pool samples

p

number of pool sample sets. Defaults to 3.

weigh

logical; if TRUE, the average expression in x_pools is reweighed by the distribution of the p pool sample sets in ref_pools.

Details

The target and reference expression samples, as well the target and reference pool samples all need to be specified. We recommend reweighing the target pool samples when calculating the average expression by the distribution of reference pools.

Value

normalized gene expression

Author(s)

Derek Chiu