new_ezmmek_sat_fit {ezmmek}

new_ezmmek_sat_fit

Description

Creates dataframe containing calibrated enzyme activity data and Michaelis-Menton fit of class 'new_ezmmek_sat_fit'

Usage

new_ezmmek_sat_fit(
  std.data.fn,
  act.data.fn,
  ...,
  km = NULL,
  vmax = NULL,
  method = NA
)

Arguments

std.data.fn

Standard data file as character string

act.data.fn

Activity data file as character string

...

User defined column names to join and group std.data.fn and act.data.fn

km

Starting value to estimate km. Default value is median of 'sub.conc' values

vmax

Starting value to estimate vmax. Default value is max activity calculated

method

Enzyme assay protocol. Must define method as 'isc' or 'ibc'

Examples

## Not run: new_obj <- new_ezmmek_sat_fit("data/tyson_std_04172020.csv",
  "data/tyson_sat_steen_04172020.csv",
  site_name,
  std_type,
  km = NULL,
  vmax = NULL,
  method = "isc")
new_obj <- new_ezmmek_sat_fit("data/tyson_std_04172020.csv",
  "data/tyson_sat_german_04172020.csv",
  site_name,
  std_type,
  km = NULL,
  vmax = NULL,
  method = "ibc")
## End(Not run)