| wildtype_mutant_pnpp {ddpcr} | R Documentation |
Plate type: wildtype/mutant PNPP
Description
A plate of type wildtype_mutant_pnpp is a subtype of
pnpp_experiment that assumes the double-positive cluster
denotes wildtype and the other non-empty cluster denotes mutant droplets.
There are two plate types that are subtypes of wildtype_mutant_pnpp:
fam_positive_pnpp and hex_positive_pnpp.
It is not recommended to use this type directly; instead you should use one
of the subtypes.
Details
Plates with this type have the following analysis steps: INITIALIZE,
REMOVE_FAILURES, REMOVE_OUTLIERS, REMOVE_EMPTY,
CLASSIFY, RECLASSIFY.
Plates with this type have the following droplet clusters:
UNDEFINED, FAILED, OUTLIER, EMPTY (double-negative),
RAIN (not empty but not wildtype nor negative), POSITIVE (wildtype),
NEGATIVE (mutant).
See the README for more information on plate types.
See Also
plate_types
fam_positive_pnpp
hex_positive_pnpp
pnpp_experiment
analyze
remove_failures
remove_outliers
remove_empty
classify_droplets
reclassify_droplets
Examples
## Not run:
plate <- new_plate(sample_data_dir(), type = plate_types$wildtype_mutant_pnpp)
type(plate)
## End(Not run)