fam_positive_pnpp {ddpcr}

Plate type: FAM-positive PNPP

Description

A ddPCR plate of type fam_positive_pnpp, which can also be expressed as (FAM+)/(FAM+HEX+), is a subtype of both pnpp_experiment and wildtype_mutant_pnpp. Use this plate type if your data has three main clusters of droplets: double-negative (empty droplets), FAM+HEX+ (wildtype droplets) and FAM+HEX- (mutant droplets).

Details

Plates with this type have the following analysis steps: INITIALIZE, REMOVE_FAILURES, REMOVE_OUTLIERS, REMOVE_EMPTY, CLASSIFY, RECLASSIFY.

Plates with this type have the following droplet clusters: UNDEFINED, FAILED, OUTLIER, EMPTY (double-negative), RAIN (not empty but not wildtype nor negative), POSITIVE (wildtype), NEGATIVE (mutant).

See the README for more information on plate types.

See Also

plate_types
wildtype_mutant_pnpp
hex_positive_pnpp
analyze
remove_failures
remove_outliers
remove_empty
classify_droplets
reclassify_droplets

Examples

## Not run: 
plate <- new_plate(sample_data_dir(), type = plate_types$fam_positive_pnpp)
type(plate)
plate %>% analyze %>% plot

## End(Not run)