custom_thresholds {ddpcr}R Documentation

Plate type: custom thresholds

Description

The custom_thresholds plate type is used when you want to gate ddPCR droplet data into four quadrants according to HEX and FAM values that you manually set. All wells in the plate will use the same threshold values.

Details

Plates with this type have only three analysis steps: INITIALIZE, REMOVE_OUTLIERS, and CLASSIFY (according to the custom thresholds).

Plates with this type have the following droplet clusters: UNDEFINED, OUTLIER, EMPTY (bottom-left quadrant), X_POSITIVE (bottom-right quadrant), Y_POSITIVE (top-left quadrant), BOTH_POSITIVE (top-right quadrant).

See the README for more information on plate types.

See Also

plate_types
x_threshold
y_threshold
thresholds
analyze
remove_outliers
classify_thresholds

Examples

## Not run: 
plate <- new_plate(sample_data_dir(), type = plate_types$custom_thresholds)
type(plate)
plate %>% analyze %>% plot

## End(Not run)

[Package ddpcr version 1.15 Index]