Eff875 {chipPCR} | R Documentation |
Highly Replicate Number Amplification Curves
Description
Highly replicate number amplification curves with an approximate amplification efficiency of 87.5 percent at cycle number 18. The data were derived from a simulation such as the AmpSim function.
Usage
data(Eff875)
Format
A data frame with 40 (Cycles) observations on the following 1000 (amplification curves) variables. The columns are all replicates.
Examples
data(Eff875)
plot(NA, NA, xlim = c(1,40), ylim = c(0,max(Eff875)), xlab = "Cycle",
ylab = "RFU",
main = "Amplification Curves with 87.5 Percent Efficiency")
apply(Eff875[, 1:ncol(Eff875)], 2, function(x) lines(1:40,x))
Fmean <- rowMeans(Eff875[, 1:ncol(Eff875)])
lines(1:40, Fmean, col = "red", lwd = 3)
legend(1, quantile(unlist(Eff875), 0.9), c("Raw", "Mean"),
pch = c(19,19), col = c(1,2))
[Package chipPCR version 1.0-2 Index]