breakage.plot {breakage} | R Documentation |
Calculates and plots a current-distance or resistance-distance relation from recorded current-position data during pipette tip breakage.
breakage.plot(x, time.limits = NULL, btm = find.bottom(x$Z), pch = 16, col = 1, lty = "solid", col.l = 2, plot.line = FALSE, f = median, mV = NA, ...)
x |
A data frame containing the experimental current and position traces. The frame should include a
column named |
time.limits |
An optional 2-element vector specifying lower and upper bounds in the |
btm |
An optional vector of division points breaking the signal into segments. Typically this is obtained
from the |
pch |
Symbol to use for plotting individual current/resistance measurements. |
col |
Colour to use for plotting the current/resistance points. |
lty |
Line type to use for plotting a regression line. |
col.l |
Colour to use for plotting a regression line. |
plot.line |
Whether to plot a regression line. By default this is not done, as a linear regression line corresponds poorly to the resistance behaviour. It may be a useful rough indicator when plotting only current data. |
f |
Function to use for determining the current between hops. By default the |
mV |
The pipette potential at which the data were recorded. If not supplied, resistance cannot be calculated and the current data are graphed directly. |
... |
Other graphical parameters to be passed to the |
Z |
The identified bottom of hop positions, in microns. |
pA |
The corresponding median (or other) current through the withdrawn pipette, in picoamps. |
Mohm |
The calculated pipette resistance in megohms. (Only present if |
Matthew Caldwell
Caldwell, M., Del Linz, S. J. L., Smart, T. G. S. and Moss, G. W. J. 2012 Method for estimating the tip geometry of scanning ion conductance microscope pipets. Anal. Chem. 84(21):8980–8984
# load the example data set data("break.data") breakage.plot(break.data, mV=100)