enzyme {asbio}

Enzymatic rate data for the phospholipase protein ExoU

Description

The bacterium Pseudomonas aeruginosa causes disease in human hosts leading to sepsis and even death in part by secreting lipases (proteins that break down lipids) into cellular environments. The protein ExoU is a phospholipase produce by particularly virulent strains of P. aeruginosa. Benson et al. (2009) measured of ExoU enzymatic activity under varying levels of the fluorescent phospholipase substrate PED6.

Usage

data(enzyme)

Format

A data frame with 10 observations on the following 3 variables.

substrate

PED6 concentration (in micromoles).

rate

enzymatic rate (nmol of cleaved of PED6 per mg ExoU).

sd

standard deviation of rate for each level of substrate.

Source

Benson, M. A., Schmalzer, K. M., and D. W. Frank (2009) A sensitive fluorescence-based assay for the detection of ExoU mediated PLA2 activity. Clin Chim Acta 411(3-4): 190-197.