CreateAssay5Object {SeuratObject}

Create a v5 Assay object

Description

Create an Assay5 object from a feature expression matrix; the expected format of the matrix is features x cells

Usage

CreateAssay5Object(
  counts = NULL,
  data = NULL,
  min.cells = 0,
  min.features = 0,
  csum = NULL,
  fsum = NULL,
  ...
)

Arguments

counts

A two-dimensional expression matrix

data

Optional prenormalized data matrix

min.cells

Include features detected in at least this many cells; will subset the counts matrix as well. To reintroduce excluded features, create a new object with a lower cutoff

min.features

Include cells where at least this many features are detected

csum

Function for calculating cell sums

fsum

Function for calculating feature sums

...

Arguments passed to other methods

Value

An Assay5 object