BuildNicheAssay {Seurat} | R Documentation |
Construct an assay for spatial niche analysis
Description
This function will construct a new assay where each feature is a cell label The values represents the sum of a particular cell label neighboring a given cell.
Usage
BuildNicheAssay(
object,
fov,
group.by,
assay = "niche",
cluster.name = "niches",
neighbors.k = 20,
niches.k = 4
)
Arguments
object |
A Seurat object |
fov |
FOV object to gather cell positions from |
group.by |
Cell classifications to count in spatial neighborhood |
assay |
Name for spatial neighborhoods assay |
cluster.name |
Name of output clusters |
neighbors.k |
Number of neighbors to consider for each cell |
niches.k |
Number of clusters to return based on the niche assay |
Value
Seurat object containing a new assay
[Package Seurat version 5.1.0 Index]