subsetBins {SQMtools}

Create a SQM object containing only the requested bins, and the contigs and ORFs contained in them.

Description

Create a SQM object containing only the requested bins, and the contigs and ORFs contained in them.

Usage

subsetBins(
  SQM,
  bins,
  trusted_functions_only = FALSE,
  ignore_unclassified_functions = FALSE,
  rescale_tpm = TRUE,
  rescale_copy_number = TRUE
)

Arguments

SQM

SQM object to be subsetted.

bins

character. Vector of bins to be selected.

trusted_functions_only

logical. If TRUE, only highly trusted functional annotations (best hit + best average) will be considered when generating aggregated function tables. If FALSE, best hit annotations will be used (default FALSE).

ignore_unclassified_functions

logical. If FALSE, ORFs with no functional classification will be aggregated together into an "Unclassified" category. If TRUE, they will be ignored (default FALSE).

rescale_tpm

logical. If TRUE, TPMs for KEGGs, COGs, and PFAMs will be recalculated (so that the TPMs in the subset actually add up to 1 million). Otherwise, per-function TPMs will be calculated by aggregating the TPMs of the ORFs annotated with that function, and will thus keep the scaling present in the parent object. By default it is set to TRUE, which means that the returned TPMs will be scaled by million of reads of the selected bins.

rescale_copy_number

logical. If TRUE, copy numbers with be recalculated using the RecA/RadA coverages in the subset. Otherwise, RecA/RadA coverages will be taken from the parent object. By default it is set to TRUE, which means that the returned copy numbers for each function will represent the average copy number of that function per genome of the selected bins.

Value

SQM object containing only the requested bins.

See Also

subsetContigs, subsetORFs

Examples

data(Hadza)
# Which are the most complete bins?
topBinNames = rownames(Hadza$bins$table)[order(Hadza$bins$table[,"Completeness"],
                                         decreasing=TRUE)][1:2]
# Subset with the most complete bin.
topBin = subsetBins(Hadza, topBinNames[1])