| Dbf4myc {Repliscope} | R Documentation |
Sequence read coverage ratios for S.cerevisiae Dbf4-9myc sample.
Description
Sequence read coverage ratios for Dbf4-9myc sample (T9394 strain). The cells were stained with DNA dye and sorted based on DNA content into S or G2/M phase fractions. Extracted DNA was sequenced and mapped to sacCer3 genome. Unique reads for replicating (S) and non-replicating (G2/M) samples were calculated in 1 kb genomic bins. The ratio was created by dividing 'score' values from replicating sample by non- replicating sample 'score' values, adjusted by total number of reads. The ratio values were further adjusted by multiplying them by 1.402 to put the values onto biologically relevant relative copy number scale from 1 to 2.
Usage
data(Dbf4myc)
Format
data frame with 11356 rows and 7 variables:
- chrom
short chromosome name
- chromStart
left chromosome coordinate
- chromEnd
right chromosome coordinate
- name.rep
replicating sample name
- name.nonRep
non-replicating sample name
- ratio
ratio value in the current bin
- ratioFactor
adjustment factor used for the current ratio
Source
S phase sample: SRA; G2 sample: SRA
References
Natsume et al. (2013) Mol Cell 50(5):661-74 (PubMed)
Examples
data(Dbf4myc)