| Myrothecium {RGCxGC} | R Documentation |
Microbial metabolism kinetics of Myrothecium sp.
Description
This object contains six chromatograms of the microbial metabolic kinetics. Myrothecium sp. were cultured in CMA. Inoculation was made from Petri dishes with the fully-grown fungal cells and sterile distilled water (to wash the surface of the plate). The plate was scraped with a sterile glass handle to obtain the spore suspension. The suspension was liquated and the concentration of 2.4 x 105 spores/mL was determined using a Neubauer chamber and an optical microscope. Then, 50 uL of the suspension was inoculated in a flow chamber into the tubes containing the culture medium. Tubes were kept at 25 celsius degree in a growth chamber with 12h of photoperiod.
A solid-phase microextraction (SPME) assay containing a DVB / CAR / PDMS (Divinylbenzene / Carboxene / Polymethylsiloxane 50/30 mm) fiber was placed into the tube headspace.
A set of columns consisting of HP-5MS 30m x 0.25mm x 0.25 um connected to a Supelcowax 1m x 0.10mm × 0.10um with a 1m x 0.25mm deactivated silica capillary being allocated between them. In these tests, a modulation period of 5s was used.
For GCxGC-QMS data acquisition, GCMSsolution version 5.3 software was used. The temperature program were 60-165 celcius at 3 celcius/min; 165 celcius - 260celcius at 20 celcius/min; 260 celcius (5 min); flow rate was 0.6 mL/min (Helium 5.0 carrier gas); splitless injection mode, ion source temperature 200 celcius, interface temperature 260 celcius; voltage 0,9 kV; mass range 50-380 m/z; acquisition rate 25Hz and electron ionization (70eV).
The original study was developed by Quiroz-Moreno et al. (2020).
Usage
data(Myrothecium)
Format
A joined_chrom object containing four slots:
- chromatograms
A named list with the two-dimensional chromatograms
- groups
The metadata containing two varaibles and six observations
- time
The retantion time range of the chromatographic run
- mod_time
The modulation time
References
Quiroz-Moreno C, Furlan MF, Belinato JR, Augusto F, Alexandrino GL, Mogollón NGS (2020). “RGCxGC toolbox: An R-package for data processing in comprehensive two-dimensional gas chromatography-mass spectrometry.” Microchemical Journal, 156, 104830.