mumu_pq {MiscMetabar}

MUMU reclustering of class physeq

Description

See https://www.nature.com/articles/s41467-017-01312-x for more information on the original method LULU. This is a wrapper of mumu a C++ re-implementation of LULU by Frédéric Mahé

Usage

mumu_pq(
  physeq,
  nproc = 1,
  id = 0.84,
  vsearchpath = "vsearch",
  mumupath = "mumu",
  verbose = FALSE,
  clean_pq = TRUE,
  keep_temporary_files = FALSE
)

Arguments

physeq

(required): a phyloseq-class object obtained using the phyloseq package.

nproc

(default 1) Set to number of cpus/processors to use for the clustering

id

(default: 0.84) id for –usearch_global.

vsearchpath

(default: vsearch) path to vsearch.

mumupath

path to mumu. See mumu for installation instruction

verbose

(logical) if true, print some additional messages.

clean_pq

(logical) if true, empty samples and empty ASV are discarded before clustering.

keep_temporary_files

(logical, default: FALSE) Do we keep temporary files

Details

This function is mainly a wrapper of the work of others. Please cite mumu and lulu if you use this function for your work.

Value

a list of for object

• "new_physeq": The new phyloseq object (class physeq)

• "mumu_results": The log file of the mumu software. Run ⁠man mumu⁠ into bash to obtain details about columns' signification.

Author(s)

Frédéric Mahé & Adrien Taudière [e-mail]

References

• MUMU: https://github.com/frederic-mahe/mumu

• VSEARCH can be downloaded from https://github.com/torognes/vsearch.

Examples

## Not run: 
mumu_pq(data_fungi_sp_known)

## End(Not run)