normalize.qpcr {MCMC.qpcr}

Internal function called by mcmc.qpcr.classic

Description

Performs multigene normalization as per Vandesompele et al 2002, centers the resulting gene expression values around the gene's means

Usage

normalize.qpcr(data, controls, center = T)

Arguments

data

The output of cq2log()

controls

Vector of control genes, such as 'controls=c("eif3h","nd5","rpl11")'

center

Whether to perform centering around mean or not.

Value

normalized dataset

Author(s)

Mikhail Matz, UT Austin

References

Matz MV, Wright RM, Scott JG (2013) No Control Genes Required: Bayesian Analysis of qRT-PCR Data. PLoS ONE 8(8): e71448. doi:10.1371/journal.pone.0071448