amp.eff {MCMC.qpcr}

amplification efficiencies and experimental Cq1 (optional column)

Description

Fold-amplification within each PCR cycle for each qRT-PCR assay. Determined by qPCR of serial dilutions of the template (see Pfaffl 2001, Nucleic Acids Res 29:00)

Usage

data(amp.eff)

Format

A data frame with 17 observations on the following 3 variables.

gene

a factor with levels actin adk c3 chrom clect eif3h g3pdh gsp2 hsp16 hsp60 hsp90 nd5 r18s rpl11 spon2 tgoln ubl3

efficiency

a numeric vector

Cq1

a numeric vector

Examples

data(amp.eff)