LDA_activity_single {LDAcoop}

LDA_activity_single

Description

calculation of clonogenic activity from data collected by a limiting dilution assay (LDA) experiment (i.e. numbers of: cells seeded, wells, positive wells).

Usage

LDA_activity_single(x, name = "cell line a", treat = "no")

Arguments

x

numeric data.frame or matrix with three columns (cells, wells, positive wells)

name

optional: experiment name (e.g. name of cell line)

treat

optional: treatment (e.g. irradiation dose in Gy)

Value

list object with estimated activity, 95 84 matrix, fit-object and p-value for cooperativity-test

Examples

x <- data.frame("cells" = c(10,50,100,250),
                "wells" = rep(25,4),
                "positive" = c(2,5,10,20))
act <- LDA_activity_single(x)
data(LDAdata)
cell.line <- unique(LDAdata$name)[1]
x <- subset.data.frame(
      LDAdata,
      subset = (name==cell.line) & (Group == 0))
LDA_activity_single(x[,4:6])