StrelkaIDVCFFilesToCatalogAndPlotToPdf {ICAMS}

Create ID (small insertion and deletion) catalog from Strelka ID VCF files and plot them to PDF

Description

Create ID (small insertion and deletion) catalog from the Strelka ID VCFs specified by files and plot them to PDF

Usage

StrelkaIDVCFFilesToCatalogAndPlotToPdf(
  files,
  ref.genome,
  region = "unknown",
  names.of.VCFs = NULL,
  output.file = "",
  flag.mismatches = 0,
  return.annotated.vcfs = FALSE,
  suppress.discarded.variants.warnings = TRUE
)

Arguments

files

Character vector of file paths to the Strelka ID VCF files.

ref.genome

A ref.genome argument as described in ICAMS.

region

A character string designating a genomic region; see as.catalog and ICAMS.

names.of.VCFs

Optional. Character vector of names of the VCF files. The order of names in names.of.VCFs should match the order of VCF file paths in files. If NULL(default), this function will remove all of the path up to and including the last path separator (if any) in files and file paths without extensions (and the leading dot) will be used as the names of the VCF files.

output.file

Optional. The base name of the PDF file to be produced; the file is ending in catID.pdf.

flag.mismatches

Deprecated. If there are ID variants whose REF do not match the extracted sequence from ref.genome, the function will automatically discard these variants and an element discarded.variants will appear in the return value. See AnnotateIDVCF for more details.

return.annotated.vcfs

Logical. Whether to return the annotated VCFs with additional columns showing mutation class for each variant. Default is FALSE.

suppress.discarded.variants.warnings

Logical. Whether to suppress warning messages showing information about the discarded variants. Default is TRUE.

Details

This function calls StrelkaIDVCFFilesToCatalog and PlotCatalogToPdf

Value

A list of elements:

• catalog: The ID (small insertion and deletion) catalog with attributes added. See as.catalog for more details.

• discarded.variants: Non-NULL only if there are variants that were excluded from the analysis. See the added extra column discarded.reason for more details.

• annotated.vcfs: Non-NULL only if return.annotated.vcfs = TRUE. A list of data frames which contain the original VCF's ID mutation rows with three additional columns seq.context.width, seq.context and ID.class added. The category assignment of each ID mutation in VCF can be obtained from ID.class column.

ID classification

See https://github.com/steverozen/ICAMS/blob/master/data-raw/PCAWG7_indel_classification_2021_09_03.xlsx for additional information on ID (small insertion and deletion) mutation classification.

See the documentation for Canonicalize1Del which first handles deletions in homopolymers, then handles deletions in simple repeats with longer repeat units, (e.g. CACACACA, see FindMaxRepeatDel), and if the deletion is not in a simple repeat, looks for microhomology (see FindDelMH).

See the code for unexported function CanonicalizeID and the functions it calls for handling of insertions.

Note

In ID (small insertion and deletion) catalogs, deletion repeat sizes range from 0 to 5+, but for plotting and end-user documentation deletion repeat sizes range from 1 to 6+.

Examples

file <- c(system.file("extdata/Strelka-ID-vcf",
                      "Strelka.ID.GRCh37.s1.vcf",
                      package = "ICAMS"))
if (requireNamespace("BSgenome.Hsapiens.1000genomes.hs37d5", quietly = TRUE)) {
  catID <-
    StrelkaIDVCFFilesToCatalogAndPlotToPdf(file, ref.genome = "hg19",
                                           region = "genome",
                                           output.file =
                                           file.path(tempdir(), "StrelkaID"))}