overview {Fragman}

Assesing several plants with an overview

Description

This function uses information from the FSA files read from storing.inds function and creates a plot to assess graphically the peaks of several plants in certain channel in order to score manually or assess the parental fragments in the case of biparentla ppulations. If you desire to create a panel you may want to take a look at overview2. The function contains several defaults in most of the arguments, please check arguments but in general.

Usage

overview(my.inds, channel = 1, n.inds = c(1:length(my.inds)), 
        xlimi=c(min(ladder),max(ladder)), ladder, channel.ladder=dim(my.inds[[1]])[2], 
        ploidy=2, dev=50, method="iter", 
        init.thresh=200, ladd.init.thresh=200, warn=TRUE, my.palette=NULL,
        env = parent.frame())

Arguments

my.inds

List with the channels information from the individuals specified, usually coming from the storing.inds function output

channel

The channel you wish to analyze, usually 1 is blue, 2 is green, 3 is yellow, 4 is red and so on

n.inds

Vector specifying the plants to be scored

xlimi

A vector containing the base pair interval where the plot should be drawn

ladder

A vector containing the expected weights for the ladder peaks that will be found the using the find.ladder function

channel.ladder

A scalar value indicating in which channel or color the ladder was read

ploidy

A scalar value indicating the ploidy of the organism to be scored

dev

A scalar value indicating the number of indexes to be used as peak separation when deciding the ladder peaks, for more details check find.ladder function

method

An argument indicating one of the 2 methods available; "cor" makes all possible combination of peaks and searches exhaustive correlations to find the right peaks corresponsding to the expected DNA weights, or "ci" constructing confidence intervals to look for peaks meeting the conditions specified in the previous arguments

init.thresh

An initial value of intensity to detect peaks. We recommend not to deal to much with unless you have highly controlled dna concentrations in your experiment

ladd.init.thresh

A value of intensity to detect peaks in the internal use of the find.ladder function. We recommend not to deal to much with it unless you identified special situations with your ladder

warn

A TRUE/FALSE value indicating if warnings should be provided when detecting the ladder

my.palette

A character vector with the colors to be used when drawing the RFU plots. If NULL it will use the programmed palette.

env

this is used to detect the environment of the user and load the result in the same environment.

Details

No major details.

Value

If rarguments are correct the function returns a list containing

$plot

Returns a plot joining the channel for the plants specified for the color desired and the peaks found by the function using the parameters specified

$nana

Returns a vector with the names of the plants specified in the function

References

Covarrubias-Pazaran G, Diaz-Garcia L, Schlautman B, Salazar W, Zalapa J. Fragman: An R package for fragment analysis. 2016. BMC Genetics 17(62):1-8.

Robert J. Henry. 2013. Molecular Markers in Plants. Wiley-Blackwell. ISBN 978-0-470-95951-0.

Ben Hui Liu. 1998. Statistical Genomics. CRC Press LLC. ISBN 0-8493-3166-8.

Examples

data(my.plants)
my.plants <- my.plants[1:10]
my.ladder <- c(50, 75, 100, 125, 129, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375)
overview(my.inds=my.plants, channel = 1, n.inds = c(1:5), ladder=my.ladder, xlim=c(200,220))
# now use:
# locator(type="p", pch=20, col="red")$x
# to click over the peaks and get the sizes in base pairs
# when you are done make sure you press the "Esc" key, 
# do not push the stop button, some versions of R usually crash 
# by stopping instead of pressing 'Esc'.