elisa_plate {ELISAtools}

Constructor function to build an elisa_plate object

Description

S3 method as a constructor to build the S4 class object of the elisa_plate elisa_plate

Usage

elisa_plate(
  batchID = NA_character_,
  expID = NA_character_,
  desc = NA_character_,
  data.std = data.frame(),
  mdata.std = data.frame(),
  data.unknown = data.frame(),
  mdata.unknown = data.frame(),
  normFactor = NaN,
  range.ODs = c(-1, -1)
)

Arguments

batchID

characters to specify the batch

expID

characters to specify experiment or plate ID

desc

characters for the data/experiment information

data.std

data.frame for standard curve data

mdata.std

data.frame containing the mean ODs and concentration of the calibration data

data.unknown

data.frame for data of samples with unknown concentration fitted with either four- or five-parameter logistic function.

mdata.unknown

data.frame containing the mean ODs and concentration by sample IDs.

normFactor

numeric the correction factor for batch effects.

range.ODs

numeric the min and max ODs in the plate.

Details

S3 method as a constructor to build the S4 class object of elisa_plate elisa_plate. Normally this is called to build an empty object with default values and then load data into it by calling loadData loadData or load.ODs load.ODs

Value

an elisa_plate object

See Also

nls.lm loadData elisa_plate load.ODs

Examples

elisa_plate();