TwoPrimerRemove {CleanBSequences}

Clean biological secuences

Description

Curates biological sequences of two restriction enzyme primers or cloning vectors.This cleaning is required for techniques such as cDNA-AFLP.

Usage

TwoPrimerRemove(SEQs, PrimerF, PrimerR)

Arguments

SEQs

DNAString containing biological sequences that are to be cleaned.

PrimerF

dnastring containing the foward primer/vector sequences to be removed.

PrimerR

dnastring containing the reverse primer/vector sequences to be removed.

Value

clean biological sequences and visualization of the alignments

Author(s)

Florencia I Pozzi, Silvina A. Felitti

Examples

SEQs = DNAString(paste("ACTTTCTGCTGCTTGTGGTCGCAATCAGAGTCCTGATGATGAGTCCTGA",
"CCGAACCCTTTTTCTCCGTCATCCGTTGGTCCATGGTACGCAATCAGAG", sep = ""))
PrimerF = DNAString("GATGAGTCCTGACCGAA")
PrimerR = DNAString("GACTGCGTACCATGC")
TwoPrimerRemove (SEQs,PrimerF,PrimerR)